It is well-known that the defects (existing in structures) and the material degradation(caused by the fatigue load) generally initiate the failure of structures. The NDE such as ultrasonic technique which can be used to detect the size and the orientation of defects has been utilized to estimate the life and stability of structures. However, only few experimental reports made by using the ultrasonic technique have been published for the case of fatigue estimation of materials and structures. The purpose of this study is, thus, to derive the relationship between ultrasonic attenuation and fatigue behavior of Polycarbonate and PMMA through ultrasonic characterization. It is also attempted to offer NDE experimental procedure which may be useful to predict fatigue life.