Forty extracted human mandibular second molars with C-shaped canal were chosen to study the anatomy of the root canal. The experimental teeth were injected with china ink, decalcified and cleared with Winter green oil, in vitro, to study the number of root, root canal, canal per root, frequency and location of lateral canal and transverse anastomosis. 1. All teedth had one root. 2. Mesial roots with two canals were 25%, and mesial roots with one canal were 75%. All distal roots had one canal. 3. In the roots with two canals, the common apical foramen appeared in 20% and the separte apical foramen appeared in 80%. 4. The frequence of lateral canal was 33.3% and the most frequent region was middle 1/3. 5. All teeth had the transverse anastomosis in all region of the roots.