- 생쥐 상실배의 초급속동결
- Ultrarapid Freezing of Mouse Morulae
- ㆍ 저자명
- 백청순,서병희,이재현,이경광,Baik. C.S.,Suh. M.D.,Lee. J.H.,Lee. K.K.
- ㆍ 간행물명
- 大韓不妊學會雜誌
- ㆍ 권/호정보
- 1990년|17권 1호|pp.81-85 (5 pages)
- ㆍ 발행정보
- 대한생식의학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
We cryopreserved mouse morulae by a simple ultra-rapid method of freezing embryos directly in $LN_2$ after holding 2min in a $LN_2$ vapor, and thawed them in $37^{circ}C$ water bath. The time requirements for permeation and dehydration by 2.0 M glycerol and 0.2 M sucrose before freezing were studied. When the embryos were equilibrated for 10 min, the optimun post-thaw survival was obtained. Embryos those developed normally to blastocyst after in vitro culture for over 24hrs were regarded as survival ones. Two experiments to assess post-thaw survival following predehydration in various mixtures of glycerol and sucrose were also accomplished. When sucrose was held constant (0.2 M) and glycerol concentration varied (1.5-3.5 M), post-thaw survival was best (78.0%) in 3.0 M glycerol. When glycerol was held constant (3.0M) and sucrose concentration varied (0.0-1.0M), optimun post-thaw survival (78.0%) was found in 0.2 M sucrose.