The distances from the center line between maxillary right and left central incisors(the dental midline) to the various anatomical landmarks were measured. Fifty five students(thirth four males and twenty one females) who have at least natural teeth including maxillary and mandibular incisors and bicuspids were examined. 1. There was statistically significant difference between the dental midline and the center line of maxillary labial frenum(p<0.05). 2. There was no statistically significant difference between the dental midline and the point of incisive papilla, philtrum line, the center line between two mandibular central incisors, and the median palatine suture line(p>0.05). 3. There was no statistically significant sexual difference among data. 4. The philtrum line showed the highest value of correspondence to the center line between two maxillary central incisors followed by the center point of incisive papilla, the center line of two mandibular central incisors, median plaltine suture line and the center line of maxillary labial frenum at the decreasing rate.