This study assessed the contribution of heavy metals to total toxicity as well as the presence other toxic compounds before and after adding the chemical P to concurrently conducted bioassay tests of Daphnia magna and P. Phosphoreum. The following conclusions were drawn from this study : With excessive EDTA dosage, a toxicity reduction in Microtox would occur due to a metal-comples being formed. Microtox was far less sensitive than D. magna to heavy metal toxicity, but extended exposure time and reagent could increase the sensitivity.