This study was designed to investigate the effects of dietary glycosyl-sucrose on blood glucose and serum lipids concentrations and carbohydrate hydrolysing enzyme activities in rats. Male Sprague-Dawley rate were divided into 3 experimental groups, cornstarch group(68% cornstarch diet), sucrose group (20% sucrose + 48% cornstarch diet) and glycosyl-sucrose group(20% glycosyl sucrose + 48% cornstarch diet). After 6 weeks, the mean values of food efficiency ratio of 3 groups were not significantly different. There were no significant differences in mean fasting blood glucose levels of three groups, while the glucose response at 30 and 60 minutes and the group as compared with sucreose area showed a tendency to decrease in glycosyl-sucrose group as compared with sucrose group. Serum lipids (T. G., phospholipids, HDL-cholesteol and total lipid) contents showed no significant differences among 3 groups but a tendency of jejunum and ileum were significantly reduced in glycosyl-sucrose group as compared with cornstarch group. The maltose hydrolysing enzyme activity, especially in jejunum was lower in glycosyl-sucrose group than in sucrose group.