A study on the effects of volumetric loading rate, surface loading rate and hydraulic. retention time (HRT) for the anaerobic treatment was conducted with the anaerobic fixed-film process using synthetic wastewater at lower temperature than that of conventional anaerobic treatment. The results are as follows 1. Alkalinity and pH value decreased as the hydraulic retention time increased 2. Increase of the volumetric lodaing rate led to increasl of effluent COD concentration and decrease of COD removal efficiency. 3. The removed volumetric loading rate increased linearly according to the increase of the volumrtric loading rate. 4. Similarly, the linear increase of the removed surface loading rate was noticed with an increase of the surface loading rate.