This experiment was carried out to determine the effects of cutting frequency and nitrogen fertilization in the mixed pasture on root production and its depth distribution. The results are summarized as follows: 1. Root distribution studied on botanical composition was not significantly different by the upper 20cm level in all treatments. 2. Root yields were all high irrespectively of dominant species. However, Arrhenatherum elatius dominant pasture showed the lowest. Alopecurus pratensis dominant pasture showed the highest in root yield. 3. With the root yield, there was no significant difference in cutting frequency, but the moderate nitrogen level(N-2) showed the highest root yield among three N levels. 4. The depth distribution of root was 1m depth in all treatments.