In order to develop an effective methods of electroacupuncture regional analgesia for Cattle, six adult Korean-native cattle, which were healthy and had ordinary perception response, were examined on the study. Using them, there were treated with six different prescription applying on six mendian points including Samyang Rack, Keud Mun, Bu Yang, Sameum Kyo, Backhyo and Hu Hae. The results are summerized as follows. 1 In the case of the acupuncture treatment on Samyang Rack and Keuk Mun located on both fore-legs. the good effect of regional analgesia was obtained toward the anterior part of cattle; on the other hand, when treated with Bu Yang and Sameum Kyo at both hindlegs. the same result were represented toward the posterior part. 2. The level of the electronic current of each electro-acupuncture treatment for bovine regional analgesia was proved to necessitated the same amount of which had given body general electro-acupuncture analgesia. 3. Any significant changes could not find out the hematological and biochemical values of all acupuncture-treated cattle during the experiment. 4. Each elector-acupuncture prescription appeared to be considerably effective for bovine regional analgesia, and also showed the possibility on applying the standing position of cattle for various surgical treatment regardless its operation areas.