This study was performed to investigate the muscular activity of the complete denture wearers compare with subjects with natural teeth. For the study, 10 subjects with natural dentition and 18 upper and lower complete denture wearers selected and the Bio-electric Processor EM2(Myo-tronics Reaserch, Inc., U.S.A.) with the surface electrodes was used to record electromyographic activity from the right and left middle of masseter and anterior temporal muscles of each subject during mandibular postural rest position, tapping of teeth from postural rest position, maximal clench, and right and left gum and raw carrow chewing. This results of this study were as follows : 1. In mandibular postural rest position, the denture wearers produces high muscular activity in contrast to natural objects(P<0.05) but, there was no difference between the state of denture removal and insertion, and the muscle activity of the anterior temporal muscle was high than the middle of masseter muscle in natural objects and denture wearers. 2. In tapping of teeth, there was no difference in muscle activity between natural objects and the state of denture removal of denture wearers. 3. In maximal clench, there was markedly lower denture wearers than natural objects in muscle activity, and the ratio of mean voltages was about 36 percentages. 4. In gum and raw carrow chewing, the activity was lower than natural object, the ratio was about 59 percentages. 5. In chewing, the mean voltages of the middle of masster muscle on the chewing side was highest, followed by the anterior temporal on the chewing side, the anterior temporal and masster muscles on the non-chewing side.