The wall shear stress in the vicinity of end-to end anastomoses under steady flow condi- tions was measured using a flush-mounted hot-film anemometer(FMHFA) probe. The experi- mental measurements were in good agreement lith numerical results except In flow with low Reynolds numbers. The wall shear stress increased proximal to the anastomosis in flow from the Penrose tubing (simulating an artery) to the PTFE graft. In flow from the PTFE graft to the Penrose tubing, low wall shear stress was observed distal to the anastomosis. Abnormal distributions of wall shear stress in the vicinity of the anastomosis, resulting from the compli- ance mismatch between the graft and the host artery, might be an important factor of ANFH formation and the graft failure. The present study suggests a correlation between regions of the low wall shear stress and the development of anastomotic neointimal fibrous hyperplasia (ANFH) in end-to-end anastomoses.