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Production and Characterization of Crystalline Cellulose-Degrading Cellulase Components from a Thermophilic and Moderately Alkalophilic Bacterium
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  • Production and Characterization of Crystalline Cellulose-Degrading Cellulase Components from a Thermophilic and Moderately Alkalophilic Bacterium
  • Production and Characterization of Crystalline Cellulose-Degrading Cellulase Components from a Thermophilic and Moderately Alkalophilic Bacterium
저자명
Kim. Dong-Soo,Kim. Cheorl-Ho
간행물명
Journal of microbiology and biotechnology
권/호정보
1992년|2권 1호|pp.7-13 (7 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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A moderately thermophilic, alkalophlic and powerful crystalline cellulose-digesting bacterium, Bacillus K-12, was isolated from filter paper wastes and found to be similar to Bacillus circulans or Bacillus pumilis, except for its ability to grow at a moderately high pH and temperature. The isolate grew at a pH ranging from 6 to 10 and at a temperature ranging from 35 to $65^{circ}C$ and produced a large amount of cellulase components containing avicelase, xylanase, CMCase, and FPase when grown in avicel medium for 5 to 7 days at $50^{circ}C$. The crude enzyme preparation from the culture broth hydrolyzed xylan, raw starch, pullulan and ${eta}-1,3$ glucan such as laminarin. Furthermore, the enzyme hydrolyzed crystalline cellulose to cellobiose and glucose and had a broad pH activity curve (pH 6~9). The enzyme was stable up to $70^{circ}C$.