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Effects of Light Pulse Intensity and Quencher Concentration on the Time-Dependent Fluorescence Quenching Kinetics
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  • Effects of Light Pulse Intensity and Quencher Concentration on the Time-Dependent Fluorescence Quenching Kinetics
  • Effects of Light Pulse Intensity and Quencher Concentration on the Time-Dependent Fluorescence Quenching Kinetics
저자명
Yang. Mino,Lee. Sangyoub,Shin. Kook Joe,Choo. Kwang Yul,Lee. Duckhwan
간행물명
Bulletin of the Korean Chemical Society
권/호정보
1992년|13권 3호|pp.325-331 (7 pages)
발행정보
대한화학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

By using the general theoretical framework proposed recently for treating the fluorescence quenching kinetics, we investigate the effect of light pulse intensity on the decay of fluorescence which follows excitation of fluorophors by the light pulse of very short but finite duration. It is seen that conventional theory breaks down when the exciting light pulse has a pulse width comparable to the fluorescent lifetime and its intensity is very high. We also find that even when the light intensity is not too high, conventional theory may fail in either of the following cases: (i) when the quencher concentration is high, (ii) when there is an attractive potential of mean force between the fluorophor and quencher, or (iii) when the energy transfer from the fluorophor to the quencher may also occur at a distance, e.g., via dipole-dipole interaction. The validity of the predictions of the present theory may thus be tested by fluorescence quenching experiments performed under such situations.