For the investigation of phosphorylation of intracellular proteins in the ginseng cells caused by the interaction between cells and an elicitor chitosan, the two cell lines (PGL 2 and PGL 3) were obtained in the suspension culture by their growth patterns. In both cell lines, de novo syntheses of cytosolic proteins were revealed to continue during incubation of inorganic phosphate, in contrast with the case of acid-soluble proteins (incubation-time independent amount of proteins). An identical incubation time (4 h) for the maximum uptake of inorganic phosphate was observed in the two cell lines. However, different times for the maximum incorporation of inorganic phosphate to the intracellular proteins were observed in terms of cell lines and types of proteins; 1) 50,992 cpm/mg protein in case of cytosolic proteins in PGL 2 at 8 h of incubation, 2) 100,000 cpm/mg acid-soluble proteins in PGL 2 at 4 h of incubation, and 3) 124,412 cpm/mg cytosolic proteins in PGL 3 at 4 h of incubation. Faster incorporation of phosphate into the acid-soluble proteins than the case of the cytosolic proteins was observed at early time range (<1 h). When the chitosan was added to the $^{32}Pi$-labelled cells (PGL 2), the phosphorylation of cytosolic proteins was fluctuated in the presence of 1 mM $[Ca^{2+}]_0$ plus 3 mM $[EGTA]_0$ in the medium. However, in the presence of 1 mM $[Ca^{2+}]_0$ only, a predominant phosphorylation of the proteins was revealed at 10 min. In contrast, EGTA effect was not appeared in the acid-soluble proteins and faster phosphorylation of the acid soluble proteins than the one of the cytosolic proteins was also observed in the presence of chitosan and 1 mM $[Ca^{2+}]_0$.