- Rhizopus nigricans의 Steroid 전환 반응에 대한 이온의 변화
- ㆍ 저자명
- 이정진,김말남,Lee. Jung-Jin,Kim. Mal-Nam
- ㆍ 간행물명
- 한국균학회지
- ㆍ 권/호정보
- 1993년|21권 1호|pp.23-27 (5 pages)
- ㆍ 발행정보
- 한국균학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
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Rhizopus nigricans에 의한 progesterone의 $11{alpha}-hydroxylation$과 ${5alpha}-reduction$반응에 미치는 이온의 효과를 조사하였다. $Cu^{2+},;Cd^{2+},;Co^{2+},;Mn^{2+},;Zn^{2+},;Fe^{2+},;Mg^{2+},;Fe^{3+}$ 및 $Na^+$은 $11{alpha}-hydroxylation$ 반응의 활성을 저하시켰으나 $K^+$은 이 반응을 촉진시켰다. $11{alpha}-hydroxyprogesterone$을 $5{alpha}-reduction$시키는 효소는 $Fe^{2+},;Mn^{2+},;Mg^{2+},;Co^{2+},;Zn^{2+},;Fe^{2+},;K^+,;Na^+$에 의하여 그 활성이 증가되었고 $Cd^{2+}$과 $Cu^{2+}$은 이 반응을 억제하였다. $11{alpha}-hydroxylation$ 반응을 촉진하는데는 $10^{-3};M$의 $K^+$이 효과적이었으며 $5{alpha}-reduction$반응을 엑제하기 위하여는 $10^{-4};M$의 $Cd^{2+}$이 적합하였다. R. nigricans는 세포내의 다효소체계에 의하여 progesterone이 $11{alpha}-hydroxyprogesterone$으로 전환된 후 $11{alpha}-hydroxy-allopregnane-3, 20-dione으로 $5{alpha}-reduction$되므로 $10^{-3};M$의 $K^+$을 먼저 첨가하여 2시간 동안 progesterone 전환 반응을 실시한 후에 $10^{-4};M$의 $Cd^{2+}$을 첨가하였을 때 가장 높은 $11{alpha}-hydroxyprogesterone$의 수득률을 얻을 수 있었다.
Ion effects on $11{alpha}-hydroxylation$ of progesterone and $5{alpha}-reduction;of;11{alpha}-hydroxyprogesterone$ by Rhizopus nigricans were investigated. Metal ions such as $Cu^{2+},;Cd^{2+},;Co^{2+},;Mn^{2+},;Zn^{2+},;Fe^{2+},;Mg^{2+},;Fe^{3+};and;Na^+$ reduced the $11{alpha}-hydroxylation$ activity, while $K^+$ stimulated the same reaction. Enzyme activity for the $5{alpha}-reduction$ of $11{alpha}-hydroxyprogesterone$ was increased in the presence of $Fe^{2+},;Mn^{2+},;Mg^{2+},;Co^{2+},;Zn^{2+},;Fe^{3+},;K^+;and;Na^+$, whereas it was decreased in the presence of $Cd^{2+};and;Cu^{2+}$. Potassium ion of $10^{-3};M;$ of concentration was found to be effective for the promotion of $11{alpha}-hydroxylation$. On the other hand, cadmium ion of $10^{-4};M$ was proved to suppress the $5{alpha}-reduction$ reaction. Progesterone is reported to be transformed into $11{alpha}-hydroxyprogesterone$ which, in turn, is converted further into $11{alpha}-hydroxy-allopregnane-3$, 20-dione by R. nigricans. From this point of view, the highest yield of $11{alpha}-hydroxyprogesterone$ could be obtained when potassium ion of $10^{-3};M$ was given initially followed by addition of cadmium ion of $10^{-4};M$ to limit conversion of 11{alpha}-hydroxyprogesterone into $11{alpha}-hydroxy-allopregnane-;3$, 20-dione.