Polymerized vesicles were prepared from monomeric cholesterol-containing surfactant. These vesicles were used to extract the free cholesterol. To introduce spacer group into [cholesteryloxycarbonylmethyl] [2-(methacryloyloxy)ethyl]dimethylammonium chloride (CHODAMA), [5-[ (cholesteryloxy)carbonyl]pentyl] [2-(methacryloyloxy)ethyl]dimethylammonium bromide (CHODAMA-5) and [10-[(cholesteryloxy)carbonyl]decyl] [2-(methacryloyloxy)ethyl]dimethylammonium bromide (CHODAMA-10) were synthesized, In both case of polymerized vesicle with CHODAMA-5 and CHODAMA-10, the permeability was decreased and the amount of extracted cholesterol was also decreased, compared with the polymerized vesicle of CHODAMA.