Polymerized vesicles were prepared from the monomeric cholesterol-containing surfactant. Spacer groups were introduced into [(cholesteryloxy)carbonylmethyl] [2-(methacryloyloxy)ethyl]dimethylammonium chloride (CHODAMA). In case of introducing the spacer groups to the cholesterol moiety, both permeability of vesicles and that of polymerized vesicles were decreased. In case of introducing the spacer groups to monomeric moiety, permeability of vesicles was increased, while that of polymerized vesicles was decreased.