Human and animals are living by acclimation to environmental changes like high and cold temperature, nose, confinement, etc. If the above changes reach a defined levels, some physiological abnormal state could appear, which we call them as stress state. Catecholamines are excreted by the sympathetic-adrenomedullary system in free from in urine. Catecholamines are derived from the adrenal medulla and urinary epinephrine can be taken as a rough estemation of the activity of this gland. Many scientist reported the endocrinological change, excretion of catecholamine and its metabolites, stomach ulcer formation, etc. under the condition of the confinement and high temperature. In this study author gave restraint, electric shock and immersion stress to rats by administrating by HPLC and got the following results. 1. In the restriant experiment, epinephrine contents in control rat was 194.7 ng, but in Bohyulanshintang administered rat urine 198.9 ng of epinephrine was found. 2. In the electrical shock experiment, 199.5 ng of epinephrine was found in the control rat urine, but in Bohyulanshintang administered rat urine epinephrine content was 142.4 ng. 3. Dopamine contents in control rat urine the immersion environment was 118.9 ng, but in Bohyulanshintang administered rat urine only 55.2 ng of dopamine was found. 4. Incontrol rat stomach there appeared focal erosion and inflamatory exudate, but in experimental group these symptom were turned to mild condition.