The objective of this study was to investigate the changes of the fabric absorbency, stiffness and antistatic property by using a fabric softner at laundering. The samples selected in this study were cotton and polyester fabrics. The cotton fabric was treated with 1/2 times(0.035%), 1 time(0.07%), 3 times(0.21%) and 10 times(0.7%) of the commercially suggested concentration (0.07%) of the fabric softner. And polyester fabric was done at 1 time(0.07%) of that. In order to examine the effect of the fabric by using a fabric softner, the abstraction of a surface active agent and quantitative analysis was performed by using a UV/VIS Spectroscopy and the correlations among the absorbency, stiffness and antistatic property was analyzed. The results obtained from this study were as follows; 1. In the cotton fabric, fabric softner gave the degradation of absorbency, and stiffness improvement was not shown by repeated fabric softner on the cotton fabric. 2. In the polyester fabric, the absorbency increased and the stiffness in creased very slightly. Specially, it is desirable to use fabric softner on the polyester due to improvement of antistatic property.