For the purpose of the precise diagnosis and proper treatment planning of obstructive jaundice, various techniques to obtain tissues from biliary strictrue sites have been proposed. We performed perutaneous transcatheter biopsies of biliary strictrues with a biotome in six patients with obstructive jaundice. The sites of biliary stricture were distal common bile ducts (n=3), common hepatic duct (n=1), and confluence of both intrahepatic ducts (n=2). Their histologic diagnoses were adenocarcinoma (n=2), chronic choledochitis (n=3), and atypical cell suspicious of malignancy (n=1). False positive or false negative results were not documented by other means (including laparotomy), when regarding atypical cell suspicious of malignancy as true positive for malignancy. Percutaneous transcatheter biliary biopsy with a biotome is easy to perform in conjunction with percutaneous transhepatic biliary drainage procedure, and can be able to obtain specific tissues for correct diagnosis.