Natural convective flow and heat transfer in a two-dimensional square enclosure fitted with a horizontal partition are investigated numerically. The enclosure was composed of the lower hot and the upper cold horizontal walls and the adiabatic vertical walls, and a partition was situated perpendicularly at the one vertical insulated wall. The governing equations are solved by using the finite element method with Galerkin method. The computations were carried out with the variations of length, position and thermal conductivity of the partition, and Rayleigh number based on the temperature difference between two horizontal walls and the enclosure height with water(Pr=4.95). As the results, an oscillatory motion of natural convection is resulted in a sudden rise of overall heat transfer, but the increase of length of partition is significantly restrained the increase of Nusselt number. The maximum heat transfer was shown just before the transition of the direction of oscillating flow. An oscillatory motion of flow was perfectly shown the stability with the decrease of the length of partition and Rayleigh number. Also, the heat transfer was raised with the increase of the thermal conductivity in proportion to the increase of the length of partition. The stability and oscillation of flow are affected by the position of partition.