Cell lines of Ginkgo biloba were derived from different plant parts and from ten varieties spanning various geographic locations. They had various properties of growth and product formation. More than three flavonol glycosides were present in low concentration in callus and suspension cultures. Cell growth and biosynthesis of flavonol glycosides were found to be affected by medium composition. Culture conditions which influenced cell growth and product formation were also examined. Light stimulated the flavonol glycosides biosynthesis and ten times higher flavonol glycosides content was obtained as compared with the result without light.