- Pseudomonas sp. DJ-12에서 분리한 2,3-Dihydroxybiphenyl Dioxygenase의 효소학적 특성
- ㆍ 저자명
- 성태경,남정현,김치경
- ㆍ 간행물명
- 산업미생물학회지
- ㆍ 권/호정보
- 1993년|21권 2호|pp.150-156 (7 pages)
- ㆍ 발행정보
- 한국미생물생명공학회
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- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
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The 2,3-dihydroxybiphenyl(2,3-DHBP) dioxygenase, the product of pcbC gene, was purified from the biphenyl and 4-chlorobiphenyl degrading Pseudomonas sp. DJ-12 by the methods of acetone precipitation, DEAE-Sephadex A-50 ion exchange chromatography, and Sephadex G-150 gel filtration chromatography. The enzyme was estimated to be about 260 kilodaltons in molecular weight and to be consisted of eight subunits. The Km value of the enzyme was 61 nM to 2,3-DHBP and the highest activity of the enzyme was observed at pH 8 and 30C.