The aims of the present study are to examine the precipitation of uranyl ion in the culture of Desulfovibrio desulfricans for the sedimentary recovery of aqueous uranium. D. desulfricans had the highest utilization rate of lactate and precipitated iron ion in the three sulfate reducing bacteria. So, subsequent experiments were conducted using lactate as an energy source. The normal growth was observed with increased pH and lactate utilization. During the culture, the amounts of SO42- consumed and S2- produced in aqueous phase were 8.5 and 7.5 mmol/m3-broth, respectively.