To investigate the effect of extracellular matrix proteins on the in vitro development of ethanol-induced parthenogenetic eggs of ICR strain mice, those were cultured in vitro in fibronectin, gelatin, or collagen precoated culture dishes containing 1.5 ml of NaH-C03$_3$-BMOC-3 medium at 37$^{circ}C$ for 96 hrs. under the atmosphere of 5% $CO_2$ and 95% air. Fibronectin, gelatin, or collagen significantly(P<O.O1) increased morula and blastocyst formation rate compared with controls in haploid (42.2, 36.8, and 38.0% vs. 4.5%), diploid (48.3, 40.9, and 43.5 vs. 4.0%), and immediately cleavaged eggs (47.1, 43.8, and 40.0% vs. 3.8%). Both the nuclear number and diameter of blastocysts developed from parthenogenetic eggs were not affected by the morphological types when they were cultured in vitro, or the kind of extracellular proteins. The nuclear number of blastocysts developed from haploid, diploid, and immediately cleavaged eggs was 45.4$pm$1.4, 45.4i1.4, and 44.8$pm$O.9, respectively. And the diameter of those eggs ranged 104.6$pm$1.9, 102.8$pm$2.3, and 103.4$pm$O.8 $mu$m, respectively.