The changes in the concentration of toropherol and carotenoid in pinenut oil at various stage of refining are studied during autoxidation. The oxidative stability of pinenut oil decreased with the refining degree increased. The tocopheol content of total crude oil was 55.51mg/100g oil and $alpha$, ${gamma}$and $eta$-tocopherol in the crude oil were 25.48mg, 23.94mg and 6.99mg in order, respectively. The amount of $delta$-tocopherol was trace. The concentrations of $alpha$, ${gamma}$ and $eta$-tocopherol in pinenut oil after degumming or alkaline refining were 23. 85mg, 19.79mg and 6.12mg or 24.08mg, 24.04mg and 5.33mg, respectively. The content of f-carotene was 0.63% of total unsaponifiable materials and that of lycopene was trace. The concentrations of carotenoids and toropherols in pinenut oil decreased while autoxidation progressed. Degrees of destruction of carotenoids and toropherols. were significant at first stage of oxidation. Decrease in $alpha$-toropherol was found to be faster than that in $alpha$, ${gamma}$-tocopherol during oxidation.