C. congregatus를 산성 액체배지(pH4.2)에 배앙할 경우 세포막연관 laccase가 배양 초기에 대량 합성분비되었으며 분비된 효소의 작용에 의하여 melanin색소가 중성배지에 비하여 빨리 생성되었고 배양 24시간 후에 배지의 산도는 pH5.2로 증가되었다. 이 효소의 합성은 transcription inhibitor인 5-fluorouracil에 의하여 크게 억제되었다.
When Coprinus congregatus was cultivated in low pH YpSs medium(pH 4.2), the culture supernatant turned brown earlier than that of normal(pH 7.1) medium resulted from the melanization synthesized by the secreted laccase reaction. The pH of medium became 5.2 after 24 h incubation. Laccase which was deeply related to the fungal development might also be implicated in the neutralization of excess hydrogen ions to protect from acidification of cytoplasm.
