This study was conducted to investigate the interaction of vitamins A and E on their transfer from diet to chicken eggs and the effect of vitamin A flood-dosing on its concentration in eggs and livers. In Experiment I, forty-two 45-wk-old brown layers (Bobeock) were divided into seven groups and fed one of seven diets: control, three vitamin A supplemented diets(8, OOO, 16, 000, and 64, 000 IU /kg diet) or three vitamin E supplemented diets (50, 100, and 200 IU/kg diet). In Experiment II, a total of thirty-two 35-wk-old white layers (Hy-ine) were divided into four groups and fed one of four diets :control, vitamin A 20, 00O+vitamin E 200 TU /kg, vitamin A 50, O00+vitamin E 200 IU /kg or vitamin A 100, OO0+vitamin E 200 lU/kg supplemented diets. In Experiment III, a total of fifty-six 35-wk-old white layers (Hy-line) was divided into four groups and fed one of four diets: control or three vitamin A supplemented diets (80, 000, 120, 000 and 160, 000 lU/kg diet). In Experiment I, vitamin E levels of egg yolk in hens fed the vitamin A supplemented diets decreased as dietary vitamin A level increased (P<O.05). On the other hand, a slight reduction in vitamin A levels of egg yolks in hens fed the vitamin E supplemented diet for 4 wk of the experiment was recovered back to control level at wk 8 (P<0.05). In Experiment II, supplemental vitamin A markedly decreased the yolk deposition of vitamin E when two vitamins were supplemented simultaneously. The yolk deposition of vitamin A decreased as high level of vitamin E was supplemented, suggesting an antagonistic interaction between the two vitamins. In Experiment III, the concentration of liver vitamin A rapidly increased and then stabilized during wk 3 to 4 after starting the experiment. Vitamin A levels in egg yolk linearly increased up to wk 6. However, vitamin A level of egg yolk in hens fed the diets supplemented 160, 000 IU vitamin A/kg was significantly lower than those of hens receiving 120, 000 IU vitamin A/kg diet (P<0.05), indicating that megadosing of vitamin A more than 120, 000 IU /kg diet be uneconomical for its enrichment of chicken eggs.