- 핵이식에의한 복제토끼 생산
- Production of cloned Rabbits by Nuclear Transplantation
- ㆍ 저자명
- 이효정,전병균,윤희준,이경미,송상현,공일근,노규진,최민철,최상용
- ㆍ 간행물명
- 韓國受精卵移植學會誌
- ㆍ 권/호정보
- 1994년|9권 2호|pp.161-166 (6 pages)
- ㆍ 발행정보
- 한국수정란이식학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
This experiment was carried out to produce cloned aniraals by nuclear transplantation in rabbits. The ovulated oocytes were collected from the oviducts between 14 and 15 hours after hGG injection. The denuded oocytes were used as nuclear recipient cytoplasm following enucleation by micromanipulation. The blastomeres separated from the 8-cell embryos were used as nuclear donor. The nucleated oocytes receiving a blastomere in the perivitelline space were electrically fused in the 0.28 M mannitol solution at 1.5 kV /cm, 60$mu$sec for three times. The nuclear transplant embryos which were used and developed to 2- to 4-cell stage in vitro were transferred into the oviducts of synchronized recipient does. A total of 64 nuclear transplant embryos were transferred to 7 recipient does and produced three offspring(4.7%) from a foster mother 31 days after embryo transfer.