The purpose of this study was to investigate the effect of nutritional status on the cell-mediated and humoral immunity in female college students. The nutritional status of twenty subjects was determined by six-days food records, anthropometric measurements, and biochemical assessments of serum nutrients. Cell-mediated and humoral immunity of the subjects was analyzed by in vivo and in vitro assessments. The results were summerized as follows : First, The average daily energy intake was 1437Kcal(CHO : PRO : FAT = 61:13:26), which corresponds to 71.9% of RDA. Anthropometric measurements showed that 50% of the subjects was under-weight(BMI<20), only 5% was over-weight(25<BMI 30), and no one was obese. Whereas the serum zinc and vitamin A concentrations underlied the normal range, iron nutritional status was relatively poor, showing 55% of the subjects was in the range of iron depletion from the measurement of serum ferritin concentration. Secondly, the total and differential WBC counts and the delayed cutaneous hypersensitivity (DCH) response of the subjects were normal. However, 15% of subjects failed to produce interleukin-2 against Con-A stimulation within 24 hours. B lymphocyte counts and immunoglobulin G, A, M levels were normal except 20% of the subjects showed low Ig A concentration. Finally, there were negative correlations between dietary fat intake and the number of positive responses in DCH. There were positive correlations between serum total protein level and T lymphocytes(CD4 and CD8), NK-cells, and total lymphocytes. Serum zinc concentration was also positively related with the number of positive responses of DCH. From the results, it could be concluded that the nutritional and immune status of the subjects were within normal range, and the nutritional status of serum protein and zinc may affect the cell-mediated immunity in human subjects.