The seepage flow has been investigated conducting laboratory experiments mainly in order to determine the relation of seepage flow friction factor against Reynolds number. The apparatus of seepage flow measurements has the water flow almost horizontaly. Several sets of experiments were carried out, and various flow conditions were obtained in each set of flow. To cover wide range of flow conditions, used were various materials of different measurement sizes and various stages of water discharge in the seepage flow tests. Shape factor equation was developed using existing data, and based on the present laboratory data, an explicit equation was developed for the estimation of friction factor of seepage flow in the range of Reynolds number from about 1 to about 600. The same equation is expected for the flow condition of Reynolds number over 600, considering the trend of friction factor distribution.