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Isolation and Characterization of an Extremely Thermophilic Sulfur-metabolizing Bacterium from a Deep-sea Hydrothermal Vent System
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  • Isolation and Characterization of an Extremely Thermophilic Sulfur-metabolizing Bacterium from a Deep-sea Hydrothermal Vent System
  • Isolation and Characterization of an Extremely Thermophilic Sulfur-metabolizing Bacterium from a Deep-sea Hydrothermal Vent System
저자명
Kwak. Yi-Seong,Kobayashi. Tetsuo,Akiba. Teruhiko,Hirikioshi. Koki,Kim. Young-Bae
간행물명
Journal of microbiology and biotechnology
권/호정보
1994년|4권 1호|pp.36-40 (5 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A water sample was taken from a black smoker chimney of a deep-sea hydrothermal vent by using an unmanned submersible "Dolphin 3K". The temperature of the hydrothermal fluid from the black smoker was $276^{circ}C$. After isolation by repeated serial dilutions, An extremely thermophilic bacterial strain was selected. The strain designated as DT1331, was an anaerobic, non-motile, coccoid shaped bacterium with about 0.5 to $1.0;mu extrm{m}$ in diameter. The strain DT1331 could grow up to $93^{circ}C$, but the optimum temperature of this strain was $80^{circ}C$. The growth occurred in the pH range of 4.5 to 8.5 and the optimum pH was 6.0. The strain DT1331 required 1% to 5% NaCl for growth and cell lysis was observed below 1% NaCl concentration. The bacterium could grow on polypeptides such as tryptone, peptone, soytone and on proteins such as casein or gelatin. However, no growth was observed on single amino acids, sugar and organic acids. Hydrogen gas was detected slightly during growth. This bacterium obligately required elemental sulfur and hydrogen sulfide gas was produced during growth.