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Production of Cellulase and Xylanase by Aspergillus niger KKS
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  • Production of Cellulase and Xylanase by Aspergillus niger KKS
  • Production of Cellulase and Xylanase by Aspergillus niger KKS
저자명
Kang. Seong-Woo,Kim. Seung-Wook
간행물명
Journal of microbiology and biotechnology
권/호정보
1994년|4권 1호|pp.49-55 (7 pages)
발행정보
한국미생물생명공학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A fungal strain capable of producing extracellular cellulase was isolated from farmland. It was identified as Aspergillus niger, and named Aspergillus niger KKS. Production of cellulase and xylanase by the A. niger KKS was studied through a shake-flask culture. The effects of culture conditions such as inoculum size, temperature, pH, and medium composition on the cellulase and xylanase production were examined. The optimum temperature and pH for the enzyme production were $30^{circ}C$ and pH 7.0, respectively. The optimized medium was composed of 2.0% (w/v) rice straw, 0.5% (w/v) proteose peptone, 0.5% (w/v) $KH_2 PO_4$, 0.05% (w/v) yeast extract, 0.01% (w/v) $CoSO_4 cdot 7H_2O$, and 0.05% (w/v) $CuSO$_4$cdot 5H_2O$</TEX>. When the strain was incubated with the optimized medium, it gave the activities of endoglucanase, $eta$-glucosidase, $eta$-xylosidase, xylanase were 3.80, 4.20, 4.00, 80.0 (IU/mL), respectively. Filter paper and cotton activities were 0.68 and 0.045 (IU/mL), respectively. The results of this study show that A. niger KKS is a potential organism with a wide spectrum of enzyme activities, such as those of $eta$-glucosidase, $eta$-xylosidase, and xylanase.