Using a model system that can compare the before and after of canal preparation in the same tooth, we measured the area of the cross section, and canal wall thickness of the distal portion of the mesial root of the mandibular molar, and compared the amount of reduction in the canal using hand flared preparation the Gates-Glidden drill flared preparation according to the changes in the MAF. The results were as follows. 1. After canal preparation, the canal wall thickness had no significant difference between the hand flared preparation and Gates-Glidden drill flared preparation. 2. The canal wall thickness, after canal preparation, there was no significant difference between the sizes of the MAF. 3. The area variation range of each cross section of root had no significant difference between MAF size and methods of canal preparation. 4. After canal preparation, the frequency of the canal wall thickness under 0.5mm showed 3.5mm below the furcation to be the most frequent with statistical significance(p<0.05). 2mm below the furcation and 5mm below the furcation followed but there was no statistical significance. 5. The danger zone of the mesial root of the mandibular molar seems to be around 3.5mm.