수수 줄기에 함유된 타감물질(他感物質)중 가장 강한 활성을 나타내는 물질을 rapid chromatography, flash flow column chromatography로 분리하고 thin layer chromatography와 HPLC로 정제하여 GC-MS로 분석하였다. Butanol, acetic acid 및 water의 용매 조합을 달리하여 타감물질을 분리한 결과 butanol (8) : actic acid (1) : water (1) 분획에서 억제효과가 가장 크게 나타났으며, flash flow column chronatography와 TLC로 분리 한 결과 가장 활성을 나타내는 물질은 Rf 0.71에서 나타났으며 HPLC로 순수분리한 결과 Rt 20.40min에서 elution되었다. 이 물질은 주황색을 띄며 methanol에 용해성이 있었다. 정제된 물질을 GC-MS로 분석한 결과 예상되는 물질은 1-methyl-1-(2-propinonyl)-hydrazine, 1-aziridineethanol, 5-chloro-2-pentanone, 2-(methylseleno)-ethanamine 중한 물질일 것으로 추측되었다.
To better understand the exact nature of the major toxic compound responsible for phytotoxicity of sorghum stem, the most toxic compound from the stem extract was isolated by rapid chromatography and subsequently purified by thin-layer chromatography(TLC) and high pressure liquid chromatography(HPLC). Of the eight fractions isolated by rapid chromatography, the fraction with solvent combinations of butanol (8) : acetic acid (1) : water (1) had the highest toxicity. Further separation of the fraction by TLC in a solvent mixture of butanol (24) : acetic acid (16.4) : water (7) : propanol (1) showed that the spot with an $R_f$ 0.71 had one major peak with retention time of 20.40 minutes. Upon subjecting gas chromatography and the HPLC fraction to the mass spectrometry, the toxic compound is probably one of the four compounds ; 1-methyl-1-(2-propynyl)-hydrazine, 1-aziridineethanol, 5-chloro-2-pentanone, and 2-(methylseleno)-ethanamine.
