본 연구에서는 녹용중에 존재하는 gangliosides 성분을 조사하였다. Folch-Suzuki 분배법에 의해 수용액층으로 분배된 당지질 성분을 Sephadex G-50 column chromatography와 투석으로 정제한 후 용매추출법을 이용하여 ganglioside 성분만을 분리하였다. DEAE-Sephadex A-25 column chromatography와 HP-TLC로 분석한 결과 녹용의 70% 에탄올 추출액에는 GM3, GM4가 주요 ganglioside이었으며 GM1과 GM2가 소량 존재하였으나, chloroform/methanol 추출물에는 GM1이 주요 ganglioside이었다. 녹용 30g중에 $0.437{mu}mol$의 ganglioside-bound sialic acid가 존재하였다.
To determine contents of gangliosides in deer antler, we separated glycolipid from deer antler according to Folch-Suzuki partition method. The glycolipid was then purified by Sephadex G-50 column chromatography and dialysis. The gangliosides were purified from the separated glycolipid by solvent extraction, 2D-HPTLC, and DEAE-Sephadex A-25 chromatography. We found that GM3 and GM4 were major gangliosides and GM1 and GM2 were minor gangliosides in 70% ethanol extract from deer antler. However, GM1 was major ganglioside in chloroform/methanol extract from deer antler. The amount of ganglioside· bound sialic acid was $0.437{mu}mol$/30g deer antler.
