Backround : To achieve optimal drug delivery rate and duration to a specific target site with desirable biodegradability of the drug carriers in the intended time period, 2% minocycline-HCl loaded microcapsule composed of alginate and chitosan were fabricated. Methods : Microcapsule were designed to maintain drug concentration in the intra-crevicular for 1 week. In vitro drug release experiments of 2% minocycline-HCI loaded microcapsules was performed in the simulated gingival crevicular fluid. In vivo biodegradability and release kinetics were evaluated by inserting 50mg of microcapsule into the pocket. The fluid concentration upto 7 days was checked by agar diffusion assay with Periopaper. And all the remnant was collected by washing technique and id biodegradability was examined by SEM. Antimicrobial activity of drug loaded microcapsules was ascertained by agar dilution and diffusion method. Cytotoxicity was investigated by MTT test to examine on the effect of drug-loaded and un-loaded microcapsule on cellular activity of gingival fibroblast. Results : In vitro and vivo drug release of the microcapsules showed zero order release kinetics with initial burst effects. The in vivo release rate was shown to $540{mu}g$ in first 24 hours and gradually diminished of $9{mu}g$ in 7days. in vivo biodegradability showed demolished shape of microcapsule at 7 days of insertion. Cytotoxicity of drug-loaded and un-loaded microcapsule showed no significant inhibitory effect on the growth of gingival fibroblast. Minocycline releasing media from 1 day to 7 days of release experiments showed sufficient microbial growth inhibitory activity. Conclusion : These results suggested that 2% chitosan-alginate microcapsule might be ideal tool for local drug delivery system for treatment of periodontal disease.