생쥐(Balb/C) 간과 신장의 microsomal cytochrome P-450 효소계에 의한 ${alpha}$-endosulfan의 시험관내 대사시험을 수행하였다. ${alpha}$-Endosulfan은 endosulfan lactone(EL), endosulfan hydroxyether(EHE), endosulfan alcohol(EA), endosulfan sulfate(ES), endosulfan ether(EE) 그리고 ${eta}$-endosulfan(${eta}$-E) 등으로 대사되었으며 주요 대사산물은 간에서 EL(13.2%) 및 EA(11.5%)이었으며 신장에서 EA(17.4%) 및 EHE(19.3%)이었다. Microsome 배양액중 유기용매 추출성 대사산물은 63.4%이었으며 수용성 대사산물은 37.1%이었다. 수용성 대사산물은 EA(83.9%), EHE(4.5%) 그리고 ES(2.3%)로서 주요 수용성 대사산물은 EA이었다. Piperonyl butoxide는 ${alpha}$-endosulfan으로부터 EE의 생성을 86%, EA의 생성을 92% 그리고 EHE, EL 및 ES의 생성을 대부분 저해하였다.
In vitro metabolism study of ${alpha}$-endosulfan by liver and kidney microsomal cytochrome P-450 monooxygenase system of the mouse(Balb/C) was performed. ${alpha}$-Endosulfan was metabolized to endosulfan lactone(EL), endosulfan hydroxyether(EHE), endosulfan alcohol(EA), endosulfan sulfate(ES), endosulfan ether(EE) and ${eta}$-endosulfan(${eta}$-E). The main metabolites of ${alpha}$-endosulfan were EL(13.2%) and EA(11.5%) in liver microsome and EA(17.4%) md EHE(19.3%) in kidney microsome. The $^{14}C$-activity of organic extractable fraction and water soluble fraction were 63.4% and 31.7% in liver micosome incubates respectively. The water soluble metabolites were EA(83.9%), EHE(4.5%) and ES(2.3). Piperonyl butoxide treatment inhibited the formation of EE by 86%, EA by 92% and EHE, EL and ES were barely formed.
