- Cellulomonas sp. KL-6에 의한 섬유소 분해효소의 생산
- ㆍ 저자명
- 정영건,권오진,Chung. Yung-Gun,Kwon. Oh-Jin
- ㆍ 간행물명
- 한국농화학회지
- ㆍ 권/호정보
- 1995년|38권 6호|pp.490-495 (6 pages)
- ㆍ 발행정보
- 한국응용생명화학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Cellulomonas sp. KL-6 균주가 생산하는 cellulase 중 CMCase나 FPase는 세포외로 다량 분비되나 ${eta}-glucosidase$는 cell bound form으로 존재하여 배양액에서의 생산은 극히 미약하였다. CMCase와 FPase는 배양 5일째에, ${eta}-glucosidase$는 배양 4일째에 각각 최대생산을 나타내었다. $CaCO_3(0.1%)$가 첨가된 효소생산 최적조건에서 CMCase는 $82;unit/m{ell}$를, FPase는 $80;unit/m{ell}$를 그리고 ${eta}-glucosidase$는 $1.2;unit/m{ell}$를 각각 생산하였고 이는 기본배지에서 보다 $60{sim}70%$의 생산증가를 가져왔다. KL-6 균주는 lignase와 laccase의 생산은 없었다.
Among the cellulases by Cellulomonas sp. KL-6. CMCase and filter paperase, which were produced as the out enzymes of cell, had been much produced, but very small amounts of ${eta}-glucosidase $, the enzyme of which is cell bound form, was produced by this organism. The optimal culture times for CMCase and filter paperase productions were 5 days, while that of ${eta}-glucosidase$ was 4 days. When this strain was cultured under the optimal medium for enzyme production, CMCase, FPase and ${eta}-glucosidase$ were $82;units/m{ell},;80;units/m{ell};and;1.2;units/m{ell}$, respectively. Thus these results were showed to increase enzyme productivities as about $60{sim}70%$ than those produced in basal medium. $CaCO_3$ injected to the medium as the ratio of 0.1% was not only enhanced cellulase activities but also effective as acid neutralizing agent. The production effects of lignase and lactase by this bacterium in filter paper medium was not appeared.