Purpose: To compare the effect of local injection therapy with absolute ethanol and hot-saline in the normalliver of rat. Materials and Methods: An experimental study was performed with the normal liver of 52 rats. Theresected livers were pathologically analyzed on three days, one week, two weeks, and four weeks after injection of0.1 ml absolute ethanol and hot-saline. The assessment was done in view of 1) main pathologic changes on time, 2)pattern of inflammatory cell infiltration, 3) measurement of necrotic area, 4) effect on vascuar and biliarytracts adjacent to necrotic area, and 5) extrahepatic peritoneal adhesion. Results: The main pathologic changeswere acute necrosis with inflammation for three days group and secondary regenerative fibrosis in all groups. Thedegree of necrosis was significantly more severe in absolute ethanol injection group, demonstrating largernecrotic area, than hot-saline injection group. The effect on vessels and bile ducts adjacent to the necrotic areawas almost not seen in both groups. The extrahepatic peritoneal adhesion was noted in both groups, but the degreewas more prominent in the absolute ethanol injection group than hot-saline injection group. Conclusion: Absoluteethanol is superior to hot-saline in the necrotic effect of percutaneous injection therapy. However, hot-salinecould be applied in case of the borderline area between mass and adjacent normal liver or the subcapsular mass.