Purpose : The in vitro dissolution of intrahepatic stones was evaluated using the various solvent mixtures.Materials and Methods : Sixty four intrahepatic stones from 16 patients were used. Four kinds of solvent mixtures(No.1 = basic buffer + EDTA, No.2 = 1 + Sulfobetain-12, No.3 = 3 + N-acetylcysteine, No.4 = 3 + urea) were used.Dissolution rates were determined by measuring the weight loss of stones after 6, 12, 24, 48 hours incubationperiods, respectively. Results : The highest dissolution rates in dissolving intrahepatic stones were achievedwith No.4 solvent mixture(1% W/V EDTA/80mM, Sulfobetain-12/1M, urea, pH 9.5). Conclusion : Intrahepatic stonescould be largely dissolved up to about 70% of their initial weight after 48 hours incubation period in vitro.