The plasmin activity of euglobulin fraction treated with earthworm extract(EWE) increased concentration dependently when EWE(0.5, 1.0 and 2.0 mg each) were added to normal plasma. It was shown that lumbrokinase in EWE may be moved to euglobulin fraction and activated plasminogen. Also anticoagulation and fibbrinolysis were studied when EWE(7.5, 30 and 120 mg/kg) were added to rat model. Prothrombin time (PT) were 19.7${pm}$ 3.8, 22.5${pm}$ 2.5 and 24.5${pm}$ 5.0sec. activated partial thromboplastin time (APTT) were 25.7${pm}$ 5.9, 28.7${pm}$ 5.2 and 36.5${pm}$ 19.1 sec. After 15 days, the production of D-dimer were 242.3${pm}$ 47.4, 250.0${pm}$ 11.9 and 205.8${pm}$ 12.2mg/ml plasma, respectively. These data showed that EWE acted on the coagulation factor of intrinsic and extrinsic system.