Cotton fabric was treated with the mixture of chitosan and DMDHEU in one step process. Effect of chitosan on dye uptake was investigated. Colorfastness to laundering and rubbing was evaluated. Performance properties including wrinkle resistance, breaking strength, and stiffness were also measured. The treated samples had an acceptable level of wrinkle resistance, yet were dyeable with direct and acid dyes. At the higher molecular weight of chitosan, the higher direct and acid dye uptakes were obtained. Reactive dye uptake under acid condition was increased appreciably compared with that under alkaline condition.