5 (sulfur)fyanoethylated keratin(SCEK), 5 (sulfur) arboxymethylated keratin(SCU) , and highcycine-tyosine keratin(HGTK) was made firm wool-keratin using nucleophilic substitution after reduction. Amino acid analysis, electrophoresis, and DSC etc. were conducted to measure the molecular weights amino acid compositions and another properties of three wool-keratin derivatives. Biodeadation behavior of these keratin derivatives was also examined in vitro by means of enzymaticestion. Two protease(papain and trypsin) were used to perform the enzymatic hydrolysis. Tensile properties of hydrolyzed keratin were measured.