The retinomotor responses of jack mackerel, Trachurus japonicus and carp, Cyprinus carpio were studied for the different strobe flash frequencies in respects of the application of the light stimulus to herding and barrier system. The strobe flash frequencies ranged at 1.7, 5, 10, 25, 430Hz in jack mackerel and 1.7, 5, 25Hz in carp. The retinal adaptation ratio was examined histologically according to the time elasped of 1, 5, 10, 30min after turning on the light in each experimental condition. Cone index were determined in order to express the retinal adaptation ratio. Jack mackerel showed an increase of the retinal adaptation ratio ranging from average 27% at the pre-stimulus light to 42.6%, 49.8%, 59.8% and 83.1% at the irradiation times of 1, 5, 10 and 30min., respectively. Carp showed an increase of the retinal adaptation ratio ranging from average 28.4% at the pre-stimulus light to 51%, 53.9% and 91.3% at the irradiation times of 1, 5 and 30min., respectively. The light adaptation process was observed to be fast approximately in order of 430Hz, 25Hz, 5Hz, 1.7Hz and 10Hz for jack mackerel, and 25Hz, 5Hz and 1.7Hz for carp, and to be light-adaptated in 30 minutes.