Effects of sulfur application on mulberry growth and soil chemical properties were examined in mulberry trees grown in a green house. Mulberry graftages were planted in Wagner pots and sulfur was applied at the levels of 0, 30, 60, or 120 kg/10a. Mulberry stem growth increased by 3.2 cm at 30 kg/ha than at 0 kg/ha sulfur application. Stem growth, however, decreased by 3.7 cm with the application of sulfur higher than at 60 kg/10a. Soil pH changed by the application of sulfur. With the application of sulfur at 120 kg/10a, soil pH decreased by 3.0. Temporal changes in the effective from sulfur content in soil indicated that sulfur dissolved between 4 to 10 days after application in the soil. Content of moisture and chemical components in mulberry leaves was also affected by the application levels of sulfur. Moisture, total-N, No3-N, K, and S content decreased, but F and Ca content increased with the application of sulfur lower than at 60 kg/10a. With the application of sulfur higher than at 60 kg/10a, P, S, and K content increased.