This study was carried out to investigate the treatment efficiency of sequencing batch reactor when the livestock nightsoil organic loading rate was varied. Sequencing batch reactor was operated with the variance of influent BOD concentration and operating cycle. The average influent BOD concentrations in this study were 150 mg/l, 200 mg/l, 250 mg/l, 300 mg/l, 350 mg/l, 400 mg/l, 450 mg/l and 500 mg/l in the condition of 1~3 cycles/day. The treatment efficiency of sequencing batch reactor is good at the volumetric loading of 0.05~0.20 kg $BOD/m^3cdot day$. Therefore, sequencing batch reactor process would become an effective alternative for the process of small scale livestock nightsoil treatment plants.