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2. 3. 5-Triphenyl Terazolium Chloride(TTC) 환원시험법의 개선에 관한 연구 : disc plate method
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  • 2. 3. 5-Triphenyl Terazolium Chloride(TTC) 환원시험법의 개선에 관한 연구 : disc plate method
저자명
정동수,김동훈,이상목
간행물명
韓國家畜衛生學會誌
권/호정보
1995년|18권 2호|pp.163-176 (14 pages)
발행정보
한국가축위생학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Triphenyl Tetrazolium Chloride(TCC) reduction test is simple and sensitive to some residual antibiotics (especially to penicillin) in milk, but comparatively insensible to sulfo-namides. The volumn of sample is also large. Thus this study was undertaken to increase the detectable level of sulfonamides in raw milk. In this study, we used small transparent plastic hole and pulp disc instead of 10m1 test tube and made test medium in which was added 0.08%TTC, 0.3% agar, 10% skim milk, approximately $10^6$ CFU/ml streptococcus thermophilus and 5ppm Trimethoprim to enhance the sensitivity for sulfonamides The results of TCC reduction test by disc plate method were summarized as follows : 1. sensitivity to residual sulfonamides were much higher than official TCC reduction test. Detectable limites of sulfamethazine, sulfamerazine, sulfathiazole, sulfachloropy-ridazine, sulfadimethoxine, sulfamononethoxine, sulfadiazine and sulfaquinoxaline were 0.1-0.5ppm levels. 2. Detectable limites to some antibiotics were simillar or good than that of official method as 0.005-0.1ppm to three ${eta}$ -lactams, 0.25-0.5ppm to one macrolide, 2-10ppm to three aminoglycosides, 0.2-0.5ppm to three tetracycline, 0.1-0.5ppm to chloramphenicol. 3. Only 0.1ml of milk was needed to test and the test medium could be stored appnoximatly 7days in the refrigerator. So test procedure was convenient than offcial method. 4. These results suggest that disc plate method is more useful to detect bacterial growth inhibition substances including sulfonamides in raw milk.