Power spectral analysis of spontaneous heart rate fluctuations were assessed by use of autooic blocking agents and changes in posture. The total power spectral range of interest is divided amongst the various experiments so that each respiratory pattern contributes a spectral ratio of interval to respiration only over a group of frequencies for which the specific respiratory pattern has substantial, and roughly constant, spectral magnitudes. System hardware is consisted ECG preamplifier, respiratory fluctuation detect, interval time generator and IBC 486PC. High frequency fluctuation, at the respiratiory frequency, are decreased by standing and are mediated solely by the parasympathetic system. Power spectral analysis is a powerful nonivsve tool for quantitying autonomic nervous system activity.