This study was carried out to investigate the effect of the concentration of nutrient solution on growth of tomato(Lrcopersicon esculentum Mill. cv. seokwang) in substrate culture. The substrates used in the experiment were perlite, vermiculite, and peatmoss. Tomato plants were treated with different concentrations of nutrient solution, viz. 0.5, 1.0, 2.0, 3.0, and 5.0mS/cm at seedling stage and transferred to different treatments, 1.0, 2.0, and 3.0mS/cm after transplanting in each substrate. Total fruit number, total yield, marketable fruit number, and marketable yield were much higher at 2.0-3.0mS/cm than at 1.0mS/cm in all three substrate culture. The percentage of malformed fruit was in order of peatmoss>perlite> vermiculite and Vitamin C content was vermiculite> perlite> peatmoss. In all three substrate culture, high marketable yield was shown when tomato plants grew with concentration of 2.0-5.0mS/cm at seedling stage and 2.0-3.0mS/cm after transplanting. The concentrations of nutrient solution after transplanting as well as at seedling stage had a great influence on total yield, marketable yield, and soluble solids. However total and marketable fruit number were considerably affected by the concentrations of nutrient solution after transplanting.